The IBD NanoBiology Facility is a new interdisciplinary core research facility for the development and application of advanced nanoscale microscopy measurement techniques to biological and biophysical systems. In addition to atomic force microscopy (AFM) scanning probe instruments, we are actively developing nonlinear confocal time-resolved fluorescence microscopies for Fluorescence Lifetime Imaging (FLIM), Fluorescence Energy Transfer (FRET), and Fluorescence Correlation Spectroscopy (FCS) in single molecular, cellular, and bulk regimes. These methodologies are excellent for noninvasive determination of phenomena such as binding, quenching, energy transfer, ion concentrations, pH, protein conformations, etc. We are also developing a Coherent Anti-Stokes Raman Scattering (CARS) confocal microscope that is sensitive to molecular vibrations, thereby enabling tag-free truly noninvasive imaging and chemical identification due to contrast mechanisms based on intrinsic molecular properties both in vitro and in vivo.