The Facility has acquired a new ChronosBH (ISS) Time-Correlated Single Photon Counting (TCSPC) spectrofluorometer that is capable of measuring fluorescence lifetimes down to 250ps in a 300-950nm spectral range with either a monochromator or discrete filters. A supercontinuum fiber laser provides 5 ps continuously tunable excitation pulses from 425 to 800nm and a complementary set of LED and laser diodes cover the UV (280, 300, 345 & 405nm). This instrument is ideal for high-resolution time-domain and spectral FRET, fluorescence polarization, and time-resolved anisotropy measurements. Long lifetimes (milliseconds) may be measured with its multichannel scaler card and accessories are available for temperature control and stop-flow.
The IBD NanoBio Facility has acquired a new Asylum Research MFP-3D-BIO atomic force microscope. This is a top-of-the-line ultra-low-noise closed-loop AFM designed specifically for biological samples. It is mounted on an inverted optical microscope for integration with simultaneous widefield fluorescence/phase/brightfield observation and has a bioheater for maintaining samples up to 80°C. It is compatible with live samples on coverslips, in Petri dishes, or in closed chambers both in air and in buffer. It is the ideal instrument for not only high-resolution imaging of DNA, proteins, cells, and tissue, but sensitive force extension and mechanical property (e.g. Young's Modulus) measurements as well. It can even do nanolithography if you'd like to write on your cells. The instrument is shared and jointly administered with the MRSEC facilities.
The IBD NanoBio Facility is pleased to announce the availability its first in-house developed instrument, a Coherent Anti-Stokes Raman Scattering (CARS) microscope. The CARS microscope is sensitive to intrinsic molecular vibrations of biomolecules and does not require the introduction of fluorescent tags to generate images. Accordingly, CARS is extremely noninvasive and can image proteins, lipids, live cells, and tissue in their native states. Additionally, CARS can also determine “fingerprint” spectra of unlabeled biomolecules that can be used for identification and localization in fixed and live samples. More information and examples can be found in the tutorials section of the reference page.
We have completed the first development stages and the CARS microscope is now producing images and data. For example, the images at right are false-color CARS images in different Raman frequency bands of an unlabeled live cell. We are now actively seeking collaborative pilot projects to establish new research directions for this exciting new instrument. Please contact the IBD NanoBio Facility if interested.